H-FABP as a serological biomarker of SKM (skeletal muscle) injury in rats has proven to be more sensitive, to have higher concordance, more positive predictive value, more negative predicted value and specificity than the established markers of SKM injury¹.

The established markers of SKM injury, creatine kinase-MM isoenzyme (CK) and aspartate aminotransferase (AST), have been useful for identifying SKM toxicity, but lack the sensitivity necessary to identify more subtle drug-related effects on SKM.

FABP3 is as reported before also a sensitive and rapid biomarker for myocardial injury². The sensitivity of FABP3 is attributed to its high concentration in heart tissue. The rapidity of its response is attributed to the small size of the protein (14kDa), which facilitates its release from the cytoplasm of damaged tissue.

The rat/mouse H-FABP ELISA (Cat.# HK403) is a reliable assay to measure H-FABP protein concentrations in serum and supernatants of tissue homogenates. This is based on spiking results and high concordance with a mass-spectrometry-based analytical method for serum¹.

See Furuhashi et al.³  for More information about FABPs.

Available reagents for research on Heart FABP: 

More information on Cell- and tissue damage

References:

1. Pritt, M et al; Fabp3 as a biomarker of skeletal muscle toxicity in the rat: comparison with conventional biomarkers. Toxicol Sci 2008, 103: 382
2. Pelsers, M et al; Fatty acid binding proteins as plasma markers of tissue injury. Clin Chim Acta 2005, 352: 15
3. Furuhashi, M et al; Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov 2008, 7: 489